The solid phase extraction technology of the Hao Zhuang (LNB) brand solid phase extraction device produced by Shanghai Haozhuang Instrument Co., Ltd.-Since the advent of the solid phase extraction method in the late 1970s, SPE columns of various stationary phases have developed very rapidly. ?? Make the solid phase extraction method continuously adapt to the needs of various samples pretreatment, and at the same time the solid phase extraction method for different types of compounds has gradually improved.
Solid phase extraction (Solid Phase Extraction) is to use a solid adsorbent to adsorb the target compound in the liquid sample, separate it from the sample matrix and interfering compounds, and then elute with the eluent or heat desorption to achieve the separation and enrichment goal The purpose of the compound. As a sample preparation technology, solid phase extraction has been more and more widely used in the laboratory.
SPE is a column chromatography separation process. The separation mechanism, choice of stationary phase and solvent are similar to high performance liquid chromatography (HPLC). However, the particle size of the SPE column packing (> 40! M) is larger than that of the HPLC packing (3 ~ 10! M). Due to the short column bed and large particle size, SPE column efficiency is much lower than HPLC column. A high-efficiency HPLC column can produce more than 10,000 trays, and 10-50 trays can be obtained from an SPE column. Therefore, SPE can only separate compounds with very different properties. In this case, instead of isocratic, a typical SPE separation is performed in a digital switching mode (digitaIon-offmode). This means that with a step gradient, the analyte is either firmly adsorbed by the stationary phase or not retained at all. Another difference from HPLC is that the SPE column is disposable.
The characteristics of the SPE column itself determine its scope of application. SPE technology with lower separation efficiency is mainly used to process samples. The objectives to be achieved with the help of SPE are: (1) remove interfering substances from the sample for later analysis; (2) enrich trace components to improve analytical sensitivity; (3) change the sample solvent to make it compatible with the analysis The methods are matched; (4) In-situ derivation; (5) Desalination of the sample; (6) Facilitate the storage and transportation of the sample.
Among the above points, purification and enrichment are the most important.
For more inquiries about solid phase extraction technology, please consult Shanghai Haozhuang Instrument Co., Ltd., senior engineers of Shanghai Haozhuang Instrument Co., Ltd. will answer your questions, the Hao Zhuang (LNB) brand independently developed by Shanghai Haozhuang Instrument Co., Ltd. Solid phase extraction device products are widely used, high stability, quality is guaranteed, welcome new and old customers to consult and buy! Reasonable price, quality guarantee, life-long maintenance, provide customers with fast, professional, high-quality systemized services, welcome your consultation buy!
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