Immunohistochemistry kit instruction manual Storage method: Store at 2-8 ℃. Validity: one year. Production date: see the seal. Working principle: The molecular weight (40000) of horseradish peroxidase (HRP), which does not cover the antigen binding site, has high activity and specificity, good solubility, the resulting product does not diffuse, and can act on a variety of bottom Substances, produce different colors and have strong HRP penetrating power, easy to enter the cell. In the presence of H2O2, DAB can be used as an electron donor for HRP, producing brown insoluble particles, and as a reagent for color development. Contents of the kit: Name Specification 1 5% BSA 15mL2 Secondary antibody 0.5 mL (undiluted) 3 H2O 2 10 mL 4 DAB chromogenic solution 10 mL 5 Citrate buffer 15ml Operation steps: 1. After anti-off tablet treatment The sections were dewaxed in xylene, and then a series of alcohol gradients were added to water, each time for 3 to 5 minutes. 2. Wash with 0.01mol / L pH7.4 PBS 3 times for 5 min each time. 3. Incubate 3% H 2 O 2 at room temperature for 5-10 minutes to eliminate the activity of endogenous peroxidase. Wash with PBS 2-3 times, 5 min each time; 4. 0.01mol / L pH6.0 citrate buffer (trachea at 80 ~ 88 ℃, other tissues 92 ℃) renature for 20min, cool to room temperature in the solution. 0.01 mol / L pH7.4 PBS was washed 3 times for 5 min each time. 5. Add the blocking solution, feel for 30 minutes in the wet box, and shake off the excess blocking solution. 6. Drop the serum onto the slice and incubate at 37 ° C for about 1 hour or overnight at 4 ° C. Wash with 0.01 mol / L pH7.4 PBS 3 times for 5 min each time. 7. Add FITC or HRP enzyme-conjugated secondary antibody dropwise, cover the slices, place the light in a humidity box at 37 ℃ for about 30 minutes, remove and wash (the same method as above). 8. DAB color development. 9. Hematoxylin light counterstaining. Dehydrated, transparent, sealed. Microscopic observation.
Outdoor Wicker Sofa Furniture
Outdoor Sofa
Furniture
Outdoor
sofa furniture is suit for outdoor garden usage for long time. The material is
UV resistant PE rattan and 1.2mm thickness aluminum frame with powder-coated.
Outstanding comfort and structural
integrity make our Outdoor Sofa Furniture Collection a welcome addition
outdoors. Rattan fibers are smoothly double woven around concealed
powder-coated aluminum frames. Gently angled backs and
wide arms encourage restful lounging. The industry`s best high-performing
fabrics encase the thick, double-wrapped foam cushions.
·
Traditional forms with a spacious, inviting scale
·
Handwoven premium resin wicker UV resistant
·
Rust-resistant powder-coated frames
·
Cushions included with 30 density sofa foam
·
Versatile tempered glass tables
·
Cushions also available with 100%
waterproof fabric.
Outdoor
sofa Furniture contain sectional sofa, deep seating sofa, round or semi-round
sofa furniture, and garden set.
If
you have any questions, please contact with us directly. Outdoor Sofa Furniture
are produced
by
Golden Eagle Outdoor Furniture With High Quality and Good Appearance. Welcome
you can visit our Factory.For any inquiry,Please send mail directly to us.
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Golden Eagle Outdoor Furniture Co., LTD. , https://www.gesunumbrella.com