Abstract: The system of high-performance liquid chromatograph consists of several parts, such as liquid reservoir, pump, injector, chromatographic column, detector and recorder. The mobile phase in the reservoir is pumped into the system by a high-pressure pump, the sample solution enters the mobile phase through the injector, and is loaded into the chromatography column (stationary phase) by the mobile phase. Different partition coefficients
1. Nutritional composition analysis
Organic acids, carbohydrates, vitamins, free fatty acids, amino acids
2. Analysis of food additives and pesticide residues
(1) Preservatives and sweeteners
Separation and determination of sorbic acid and benzoic acid preservatives in concentrated fruit juices, soft drinks, jams, oyster sauce and margarine, as well as analysis of aspartame and saccharin in cola drinks.
(2) Synthetic pigment
(3) Residues of pesticides and antibiotics
High performance liquid chromatography can be used for the determination of pesticide residues and antibiotic residues in foods such as drinking water, milk, beer, cereals, potatoes and pork.
(4) Food toxins
High performance liquid chromatography is used to determine food toxins such as polyamines, hypoxanthine, aflatoxin, and saxitoxin in soybean, fish and shellfish.
Nutritional component analysis liquid chromatography Jilin, food additives and pesticide residue analysis Liaoning, nutritional component analysis liquid chromatography Anwei, synthetic pigment analysis special liquid chromatography Heilongjiang, pesticide and antibiotic residue analysis liquid chromatography Tianjin, food toxin detection analysis liquid phase Chromatography, high-performance liquid chromatography principle: high-performance liquid chromatography is to use a high-pressure infusion pump to pump a single solvent with different polarities or mixed solvents with different proportions, buffers, and other mobile phases into a chromatographic column equipped with a stationary phase. The valve is injected with the test product and brought into the column by the mobile phase. After the components in the column are separated, they enter the detector in sequence, and the chromatographic signal is recorded by the recorder or integrator.
3. General requirements for instruments
The instrument used is a high-performance liquid chromatograph. The packing of the chromatographic column and the composition of the mobile phase should be in accordance with the regulations under each item. Commonly used packing materials for the column include silica gel and chemically bonded silica gel. The latter is most commonly used with octadecylsilane-bonded silica gel, followed by octyl-bonded silica gel, and cyano- or amino-bonded silica gel is also used; ion exchange fillers are used for ion exchange chromatography; gel or glass microspheres, etc. Size exclusion chromatography, etc. The injection volume is usually a few microliters. Unless otherwise specified, the column temperature is room temperature and the detector is an ultraviolet absorption detector. When using an ultraviolet absorption detector, the flow used corresponds to the ultraviolet spectrophotometry.
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